Chrometra Covalent Nucleic Acid labeling range:
Method Specific Information:
M-Taze enzymatic DNA modification
Methyltransferase enzymes are a subclass of enzymes which catalyze the transfer of a methyl group to a substrate, with this target ranging from small molecules over proteins to oligonucleotides. In this labeling reaction, the majority of methyltransferase enzymes use S-Adenosyl-L-Methionine (SAM, Adomet) as the methyl donor. In recent years, several of the enzymes have been shown to tolerate extended functional groups on the SAM substrate, catalyzing the transfer of larger groups to their target. This enzyme flexibility, combined with its natural substrate specificity, makes MTase based technology an exciting entry in the field of biomolecule labeling.
Currently two recognition sequences are offered, 5’-TCGA-3’ and 5′-GGATG-3′, where in both cases the adenine base is alkylated.
Chrometra Attag, general and versatile polynucleotide labeling
The alkylation of DNA nucleobases through reactive chemical species is a general method for the labeling of all polynucleotides. In this scheme, predominantly guanine nucleobases (G) are targeted by the reactive chemical agents, while the other bases are functionalized to a lesser degree. Through control over the equivalents of labeling agent and the labeling time, the density of labeling can be monitored.
The Chrometra Attag kits provide an efficient and cost-effective solution for the single step tagging of all nucleotides, while the underlying chemistry is robust and fully scalable. While predominantly used for the fluorescent visualization of DNA (e.g. In situ hybridization or microscopy) or DNA transfection experiments, the flexibility of the Attag Kits allow you to label any polynucleotide in a wide range of applications
The X-Change kit brings you optimized cytosine labeling with fluorescent dyes or capture groups. The technique requires unhybridized nucleobases for efficient transfer, and is therefore best suited for single stranded nucleic acids or short double stranded sequences. Exceptionally dense labeling can be achieved through control of reaction time and reagents.
Chrometra Opra Range
DNA intercalation by dyes is a standard method for the visualization of the nucleotide. From the wide range of intercalating dyes available, the Chrometra Opra dye set offers a comprehensive and cost-effective solution for most applications, such as cell viability assays and the analysis of single molecules of DNA. The Opra dyes are selected from monomeric or dimeric products for brightness and complex stability.
The dyes are offered as DMSO solutions, and are formulated for long-term stability. All fluorescent dyes are prone to slow degradation in diluted solutions, a process accelerated by repeated freeze-thaw cycles and exposure to light and oxygen. To reduce this effect, the preparation of aliquots and frozen storage is recommended. Chrometra also offers predispensed dyes in solid form, for the rapid preparation of fresh solution.
Chrometra Product Selection Table:
|Chrometra Product||Technology||Sequence specific||Density||Suited for||Example applications|
|Chromatre Attag||Alkylation (G)||No||Low-moderate||DNA, RNA, LNA, PNA||Transfection, Fluorescent labeling, microscopy|
|Chrometra X-Change||Alkylation (C)||No||Low to very high||RNA, ssDNA,||FISH probes, microarrays|
|Chrometra Addapt||Alkylation (G)||No||Low-moderate||DNA, RNA, LNA, PNA||FISH probes, microarrays|
|Chrometra M-Taze||Alkylation (A)||Yes||Moderate||DNA||Genetics|
|Chrometra Opra||Electrostatic/ p-p||Limited||High||DNA, RNA||DNA Assays, cell stains|