Selective functionalization of proteins essential in the elucidation of protein function and structure in a dynamic biological environment. Chemical labeling of proteins can provide detailed insights in protein-protein interaction, and allows interactions and structure to be observed in vivo.
In the tagging of proteins, care must be exerted that tertiary structure is not lost in the process, as this may for example cause enzymes to lose functionality or lead to a reduction of antibody specificity.
Reactive handles on proteins most commonly exploited are amines and thiol groups. Amines are subject to acylation and alkylation, and this mostly on lysines. Free cysteine thiols on proteins are rarer, but thiols can be generated by reducing disulfide bridges, though this may impact on the tertiary structure.
Chrometra holds extensive experience in the development of protein functionalization tools, in fluorescent labeling, specific capture and immobilization, and offers some of the most versatile and broadly applicable methods for scientific use.